These contaminations can be removed by physical,. 305–315, 1990. , Drobiova, H. Appl Environ Microb,, 70:Al-Saleh E, Drobiova H, Obuekwe C. Jacobson, and R. From the previous results was concluded the following. Samples were incubated in flasks on shaker at 120 rpm for 5 days at 22°C. · 2.
Isolates has given the most potent crude oil degrading activity were identified as Pseudomonas aeruginosa, Coronobacter sakazakii, klebsiella oxytoca and Bordetella bronchispetica consequently. Fifty-one culturable marine hydrocarbonoclastic bacteria were isolated from the Lombok Strait (LS-3, LS-13, LS-14, LS-15, LS-16 and LS-20) and Indian Ocean (IO-1, IO-6, IO-8, IO-19, IO-24 and IO-25). 2 Kaplan CW, Kitts CL. 145–151, 1995. 02 CaCl2, 1. View at: Google Scholar See in References.
· In the traditional method, the major species of phenanthrene-degrading bacteria found was Pseudomonas (Gammaproteobacteria). In this study, the efficiency of crude oil degradation of the individual bacterial cultures and the designed bacterial consortium was determined qualitatively by estimating the consumed hydrocarbons after biodegradation and by estimating the dry weight of the three bacterial isolates and. River Nile water and soil samples were collected and examined for isolation of oil degrading bacteria. , Seasonal Incidence of Some Heterotrophic Aerobic Marine Bacteria in an Avicennia marina Habitat along a Qatari Coast, Journal of Biological Sciences, 10.
The 16S rRNA gene analysis shows that the isolate obtained from the automobile workshop is of the genus P. 5 Enumeration, isolation and identification of bacteria in soil The culturable total heterotrophic (THB) and crude oil-degrading bacteria in contaminated and uncontaminated soils were counted before and after the amendments experiments predominant culturable crude oil-degrading bacteria in the coast of kuwait in order to study the effects of nutrients amendments on the THB and crude oil-degrading bacterial populations. ,, Radwan et al. · AL-Sale E, Drobiova H, Obuekwe C () Predominant culturable crude oil-degrading bacteria in the coast of Kuwait. 4g, the ratio of redox initiator is 3:2, in which.
CAS Article Google Scholar. Predominant culturable crude oil-degrading bacteria in the coast of Kuwait. Presence of polycyclic aromatic hydrocarbons (PAHs) in soil and water is major problem as environmental contaminants and most of these PAHs are recalcitrant in nature. 0 K2HPO4, 1. Al-Saleh, E. Therefore, bacteria have been screened and utilized to degrade waste products produced by the food, agricultural, chemical and pharmaceutical industries.
05 FeCl 3, and the pH adjusted to 7–7. · Al-Saleh E, Al-Drobiova H, Obuekwe C () Predominant culturable crude oil-degrading bacteria in the coast of Kuwait. Bacterial growth was measured by using spectrophotometer (Chemito Instruments UV 2600) at 600 nm and compared with control with. (v)Finally, the selected bacterial isolates could be effective in clearing oil spills and the mixed bacterial culture can efficiently degrade the crude oil components. 2 Detection of crude oil degradation efficiency of strains. However, some surfactants, such as Corexit 9500, were reported to exhibit adverse impacts on oil-degrading bacteria (Kleindienst et al.
AL-Saleh, H. Drobiova, and C. aeruginosa with reference to ATCC 27853. Fathepure, “Biodegradation of BTEX at high salinity by an enrichment culture from hypersaline sediments of Rozel Point at Great Salt Lake,” Journal of Applied Microbiology, vol.
5% of Egyptian crude oil after 28 days of incubation. Microbial predominant community of cyanobacteria mats in the intertidal zone of oil-polluted coast of Saudi Arabia. The oil degrading bacteria and other microorganisms degraded the chemical substance this process is termed as bioremediation. Leahy and R. 0 K 2 HPO 4, 1. Biodegradation, 63(4): 400–406. · Predominant culturable crude oil-degrading bacteria in the coast of Kuwait Al-Saleh, E; Al-Drobiova, H; Obuekwe, C Effect of bioremediated soil on growth of different plant species. 0 KH 2 PO 4, 1.
Colwell, “Microbial degradation of hydrocarbons in the environment,” Microbiological Reviews, vol. Oysters were carefully culled and rinsed using sterile 0. ENRICHMENT OF OIL-DEGRADING BACTERIA. Physical and chemical methods like volatilization, photooxidation, chemical oxidation, and bioaccumulation 2 1. Alvarez HM, Silva RA, Herrero M, Hernández MA, Villalba MS () Metabolism of predominant culturable crude oil-degrading bacteria in the coast of kuwait triacylglycerols in Rhodococcus species: insights from physiology and molecular genetics. and AL-Deeb, T. 1%, v/v) was injected into BH media, and 1. Obuekwe, “Predominant culturable crude oil-degrading bacteria in the coast of Kuwait,” International Biodeterioration and Biodegradation, vol.
Int Biodeterior Biodegrad 63(4):400–406 Article CAS Google Scholar. International Biodeter Biodegr. Preferential degradation of aromatic hydrocarbons in kerosene by a microbial consortium. The analysis of the 16S rDNA sequences of isolated bacteria revealed the predominance of six bacterial genera: Pseudomonas, Bacillus, Staphylococcus, Acinetobacter, Kocuria and Micrococcus. 7% by Bacillus subtilis, and 74. from seven locations along the coast of Kuwait. aeruginosa, B.
Some species of bacteria were also isolated in the microbial oil survey technique 8. Currently the world uses lots of technology, where enhanced recovery processes, desulfurization and denitrification of oil are initiated and controlled by microorganisms. Xi-51-5-1: 38°41′, N; 117°22′, E) in Dagang oilfield, near Bohai Sea, southeast of Tianjin in the northeast China. The analysis of the 16S rDNA sequences of isolated bacteria revealed. 5%) for the studied crude oil at 22°C after 28 days of incubation followed by Ps.
–,. 02 CaCl 2, 1. ZP1 and Tistrella sp.
PDF | Petroleum contaminations cause extensive damage to the ecosystem of contaminated regions. 2 were amended with 1% filter sterilized crude oil (v/v) according to Liu et al. On the other hand, the presence of bacteria and other microorganisms in deposits and transmission systems and in stored products is a very. 85% NaCl to remove debris and shell biofilm. 05 FeCl3, and the pH adjusted to 7–7. 0 g/L (w/v) of single bacteria was inoculated.
Phenanthrene utilization by bacteria with SSMS was higher than traditional cultivation method. 2 MgSO4, 0. Capillary gas chromatography was used for testing the effect of those bacterial species on the biodegradation of crude oil.
Eleven strains were selected from among the 25 isolated strains for further study based on their high growth rates on crude oil and high levels of hydrocarbon degradation. variabilis showed the most inaptitude. In recent years, the use of bacteria to deal with environmental pollutants has become a promising technology because of its low cost and eco-friendly nature ( Guerra et al. View at: Publisher Site| Google Scholar See in References.
Can bacteria degrade waste products? Bacosa H, Suto K, Inoue C. Bacterial strains (Pseudomonas aeruginosa,Bacillus subtilis, andAcinetobacter lwoffi) used in this study were identified in many ecological studies of Leahy and Colwell 11 1. International Biodeterioration & Biodegradation,63(4), 400-406. 6 AL-Saleh E, Drobiova H, Obuekwe C. , & Obuekwe, C. · In this survey, 25 crude-oil-degrading bacteria were isolated from marine environments in Iran, including the Persian Gulf and the Caspian Sea. Google Scholar.
Kuwait crude oil was used to conduct bioremediation screening of petroleum. Int Biodeterior Biodegradation 63:400–406. Bacterial succession in a petroleum land treatment unit J. Is petroleum hydrocarbon degradation bacteria? 2 were amended with 1% filter sterilized crude. This was followed by 77.
View at: Publisher Site| Google Scholar See in References among hydrocarbon degrading microorganisms. Individual bacterial cultures showed less growth and degradation than did the mixed bacterial consortium. · Previous studies based on culture-dependent techniques on Kuwait soils demonstrated the presence of culturable indigenous oil-degrading bacterial genera such as Bacillus, Paenibacillus, Pseudomonas, Rhodococcus and Streptomyces (AL-Saleh et al. Prior to collection of oyster tissues (OT), mantle fluid (MF) from each oyster was aseptically collected by opening each oyster from the hinge side and aspirating the fluid from the mantle cavity by using sterile syringes fitted with 21 gage needles. Int Biodeterior Biodegrad 63:400–406. Malkawi, H.
It is not a new concept in fact it has been studied by many Microbiologists in the 1940s but however, it became known in late 1980s as a technology for cleaning up of shorelines which are contaminated with spilled oil. aeruginosa exhibited proficient degrading ability on crude oil and spent engine oil at all days of incubation, E. An predominant culturable crude oil-degrading bacteria in the coast of kuwait experimental study was undertaken to assess the efficiency of Pseudomonas aeruginosa, Bacillus subtilis, and Acinetobacter lwoffi isolated from petroleum contaminated water and soil samples to degrade crude oil, separately and in a mixed bacterial consortium. , Drobiova H. , Olumagin A. The mineral salt media (MSM) with the following composition (g/L): 0.
predominant culturable crude oil-degrading bacteria in the coast of kuwait The results demonstrated that the selected bacterial isolates could be effective in biodegradation of oil spills individually and showed better biodegradation abilities kuwait when they are used together in mixed consortium. The crude oil (0. Isolation and selection of crude-oil degrading bacteria. · For isolating and screening crude oil-degrading bacteria, the sample of oil-water mixture was collected from the oil production well (NO. 2 AL-Saleh E. ,, Obuekwe et al.
0 NH 4 NO 3, and 0. By enrichment, domestication, and separation of culture from the soil sample of Qianjiang Guanghua Oilfield, the pure culture of three petroleum degrading bacteria G-40, G-53, and G-94 was identified from the medium supplemented with oil, which served as the sole source of carbon. Int Biodeterior Biodegrad. (ii)The individual bacterial strains have biodegradation abilities less than their combination (consortium) because the hydrocarbon mixtures differ markedly in volatility, solubility, and susceptibility to degradation and the necessary enzymes needed for biodegradation cannot be found in a single organism. In order to obtain high molecular weight polyacrylamide coagulant, preparation of the initial reaction temperature, dose of the azo initiator agent, ratio of different redox initiator agent, pH and other affecting factors were predominant culturable crude oil-degrading bacteria in the coast of kuwait investigated and the results show: the best initial reaction temperature is 15 °C, the mass of azo initiator is 0. Gao, “Isolation and characterization of phenanthrene-degrading strains Sphingomonas sp. Crude oil which used in this study was obtained from Egyptian Petroleum Research Institute. · While P.
Al–Saleh E, Drobiova H, Obuekwe C () Predominant culturable crude oil–degrading bacteria in the coast of Kuwait. Int Biodeter Biodeg 63: 400–406. , Obuekwe Ch. Int Biodeter Biodegr 63:400–406 Google Scholar. coli and C. : Effects of waste drilling fluid on bacterial isolates from a mangrove swamp oilfield location in the Niger Delta of Nigeria.
, Fatmi, L. Int Biodeter Biodegr,, 63 (4): 400-406. subtilis, and A.
AL-Saleh E, Drobiova H, Obuekwe O () Predominant culturable crude oil-degrading bacteria in the coast of Kuwait. 0 NH4NO3, and 0. · Till date microbial community studies in oil-polluted sites have been carried out either for crude oil contamination (marine and coastal sites) 14–16 or petroleum oil contamination 17, 18 or desert soils 19, 20 but limited efforts have been made in the direction of studying role of bacterial community at sites tainted with edible oil. (iii)The mixed bacterial culture could carry out a maximum degradation (88.
AL-Saleh E, Drobiova H, Obuekwe C. ,, Obuekwe et al. ZP5,” Journal of Hazardous. Request PDF | Predominant Culturable Crude Oil Degrading Bacteria in the Coast of Kuwait | Total of 272 crude oil-degrading bacteria were isolated from seven locations along the coast of Kuwait. 2 MgSO 4, 0. (iv)Pr/Ph, n-C17/Pr, and n-C18/Ph ratios in the petroleum pollutants have been traditionally used to interpret the extent of biodegradation. Twelve isolates were found to degrade crude oil efficiently at a >2% concentration and predominant culturable crude oil-degrading bacteria in the coast of kuwait to grow with crude oil as predominant culturable crude oil-degrading bacteria in the coast of kuwait their sole carbon and energy source.
Oil spillage and oil pollution in water environment have been a major threat to the ecosystem and human being through the transfer of toxic organic materials including polycyclic aromatic hydrocarbons (PAHs) into the food chain 1 1. Finally, there are many advantages has been gained from the oil degrading bacteria’s from a quick cleanup of an oil spill. Int Biodeterior Biodegradation 63:400–406 predominant culturable crude oil-degrading bacteria in the coast of kuwait CAS. Predominant Culturable Crude Oil Degrading Bacteria in the Coast of Kuwait. Al-Saleh E, Drobiova H, Obuekwe C () Predominant culturable crude oil-degrading bacteria in the coast of Kuwait.
8% by Pseudomonas aeruginosa, 76. (i)All studied strains have a great effect on the degradation of paraffinic hydrocarbons and UCM in the crude oil under study. By contrast, the major phenanthrene-degrading bacteria observed using SSMS were Sphingomonas (Alphaproteobacteria). 3 Benka-Coker M. View at: Google Scholar See in References and Al-Saleh et al.
400–406,. Luthy, “Biodegradation of naphthalene in aqueous nonionic surfactant systems,” Applied and Environmental Microbiology, vol. 3% by Acinetobacter lwoffi.
At temperature 22°C, the mixed bacterial consortium degraded a maximum of 88. In view of this, the selection. · Crude oil degrading bacteria were carried out under aerobic condition with crude oil as sole source of carbon. See predominant culturable crude oil-degrading bacteria in the coast of kuwait full list on hindawi.
Crude oil degrading bacteria were carried out under aerobic condition with crude oil as sole source of carbon. () Mutational Analysis of Oil Degrading Genes in Bacterial Isolates from Oil Contaminated Soil at the Jordanian Oil Refinery. World Applied Sciences Journal, 6, 208-220. Int Biodeter Biodegr 63:400–406; Al-Thukair A, Abed R, Mohamed L. Mar Pollut Bull. , b) because of toxicity of the surfactant toward bacteria or as a result of competition of the surfactant with hydrocarbon substrates (Laha and Luthy, 1991; Liu et al. : Predominant culturable crude oil-degrading bacteria in the coast of Kuwait, International Biodeterioration and Biodegradation,, 63: 400–406. · AL-Saleh E, Drobiova H, Obuekwe C () Predominant culturable crude oil-degrading bacteria in the coast of Kuwait.
Predominant culturable crude oil-degrading bacteria in the coast of Kuwait J. ; 63: 400-406. 0 KH2PO4, 1. In this research 18 oil-degrading marine bacteria isolated predominant culturable crude oil-degrading bacteria in the coast of kuwait from oil-contaminated seawater from the Bay of Tumaco, on the Colombian Pacific coast, were used for detecting the phaC gene and. 670, 1, 7,, (). What is oil degrading bacteria? Although oil pollution is difficult to treat, petroleum hydrocarbon-degrading bacteria have evolved as a result of existing in close proximity to naturally occurring petroleum hydrocarbons in the environment.
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